Sanger sequencing

Regular Sequencing

  • Normal Sequencing
  • Difficult Sequencing
  • Additional Services

Special Sequencing

  • Microbial Identification
  • Full Sequencing
  • Mitochondrial DNA sequencing
  • Bisulfite sequencing
  • Fragment Analysis
  • Cell line authentication
  • NGS Integration service
  • Designed PCR Sequencing (DPS)

Universal Primer list

Vector-Binding Primer

Troubleshooting

주문

일반문의

Bisulfite sequencing

This service effectively analyzes the methylation frequency in CpG islands of specific genes.

Service Details

  • The presence or absence of methylation at specific sequences is analyzed through bisulfite conversion, PCR, TA-cloning, and sequencing.
  • The degree of methylation at specific sites is assessed by frequency through multiple TA-clone sequencing results.

Workflow

STEP 1

gDNA
extraction

STEP 2

Bisulfite
conversion

STEP 3

PCR
CpG island

STEP 4

TA-Cloning

STEP 5

Sequence Analysis