코스모진텍 서비스

  • Bacterial Expression

해외 서비스

  • Bacterial Expression
  • Mammalian Cell Expression
  • Baculovirus Expression
  • Yeast Expression

주문

일반문의

Yeast Expression

분비 발현과 세포 내 발현을 모두 하는 경제성이 높은 진핵생물 발현 시스템입니다.
원핵생물 발현 단백질보다 안정적이며 특히 진핵생물 유전자의 발현 및 기능성 단백질의 제조에 적합합니다.
발현된 exogenous protein을 세포외기질로 분비하여 고순도의 단백질을 쉽게 얻을 수 있습니다.

서비스 장점

  • 저비용 및 높은 발현 수준
  • 자체 Endotoxin-free
  • 효율적인 단백질 folding 및 분비 발현
  • 간단한 배양 조건 및 정제 작업
  • 광범위한 번역 후 변형(PTM): Glycosylation, phosphorylation, acyl lipid
  • 단순한 무기염, 대형 바이오매스를 사용하여 고밀도 발효를 개발 가능
  • 원핵생물 발현보다 안정적이며, 진핵생물 유전자의 발현과 기능 발현 단백질의 준비에 적합

활용 분야

  • Purified protein (structure, enzymology, drug discovery)
  • Industrial strain improvement, amplification

Service Process

  • Steps Project Process Cusabio Features
    1 Plasmid
    Expression vector construction     		Codon optimization; gene synthesis Multiple vectors optimization, More options for customers

    In order to improve the success rate of expression and achieve higher yield, in addition to conventional N-terminal fusion protein expression, we also provide protein with C-terminal fusion label, in greater degree to ensure the activity while ensuring the purity.
    The PCR amplification products are ligated to the expression vectors e.g. pPic9k, pPic3.5k, pPiczαA, etc.
    Transform ligation mixtures into E. coli strain
    Obtain the correct recombinant plasmid
    2

    Transformation and strain identification     		Prepare the recombinant plasmid in large quantities High copy screening

    Conduct multiple screening through unique screening markers of different vectors, and the highest expression level strain was gradually obtained.     		PickRight Technology

    The high expression level strain was obtained directly after transformation, and the time was shorten by 5-10 business days compared with the traditional screening. (Theoretically this technology is mainly recommended for the production of less than 5 mg/L protein expression)
    Transfect recombinant Bacmid DNA into insect cells to obtain baculovirus, and detect expression level by SDS-PAGE; Repeat the infection if necessary
    3
    Small test, scale up expression and purification     		Small scale expression screening (20-40 strains)
    Determine strain and optimize expression conditions
    Scale up culture
    Protein purification Multiple purification methods (optional)

    Explore different chromatographic conditions including ion exchange, hydrophobic and others by using AKTA, and then determine the optimal purification method.
    4
    Additional services (Optional)     		Charge Tag removal service Flexible additional services

    Customers can flexibly choose from a variety of additional services to their specific needs, e.g. Endotoxin removal, Filter-sterilization, Tag removal, Lyophilization, etc. Some are complimentary, and some require additional charge.
    Free Filter-sterilization;
    Endotoxin removal;
    Lyophilization
    (Note: Lyophilization and Filter-sterilization can not be met simultaneously)
    5
    Quality Control     		Testing of purity, concentration, etc.
    QC report is provided.    		 Detailed COA report

    Detailed product data sheet and COA are provided for each project.