코스모진텍 서비스

  • Bacterial Expression

해외 서비스

  • Bacterial Expression
  • Mammalian Cell Expression
  • Baculovirus Expression
  • Yeast Expression

주문

일반문의

Baculovirus Expression

안전성이 높은 진핵생물 발현 시스템입니다. 높은 분자량의 단백질을 동시에 발현시킬 수 있으며 단백질 발현 후 가공과정을 통하여 원래의 형태와 유사한 단백질을 얻을 수 있습니다. Transfer vector, baculovirus vector 및 host cell로 구성되며 바이러스 백신 개발 (ex - 인플루엔자 백신, HPV 백신, 세포 신호 전달 단백질 및 cytokine, kinase 개발) 등에 사용됩니다.

서비스 장점

  • Large gene fragment 운반 능력으로 large protein 발현 가능
  • 높은 안전성 : Baculovirus는 엄격한 종 특이성으로 활성이 제한적
  • Post-translational modification을 통해 높은 생물활성을 가진 단백질을 효율적으로 발현
  • 증폭이 쉽고 대규모의 recombinant protein을 생산할 수 있음
  • Unique bacmid based expression system : 기존 방식보다 낮은 가격, 대용량 suspension transfection, 높은 titer

활용 분야

  • Purified protein (structure, enzymology, drug discovery)
  • 바이러스 백신 개발
  • Cell signaling protein
  • Cytokine, Kinase, etc.

Service Process

Plasmid

STEP 01 Plasmid Construction
플라스미드 구축
Process
  • Codon optimization; gene synthesis
  • The PCR product is ligated to the expression vectors e.g. pFastbac1-KHM, pFastBac1-MBP, etc.
  • Transform TOP10 E.coli competent cells
  • Obtain the correct recombinant plasmid
Cusabio Features
Vector optimization

In order to improve the success rate of expression and achieve higher yield, we provide protein expression using C-terminal fusion tags in addition to conventional N-terminal tags, which retains the bioactivity of the protein while ensuring high purity.

STEP 02 Preparation of recombinant Bacmid
and high titer virus
재조합 Bacmid 및 고역가 바이러스의 제조
Process
  • Transform DH10Bac cells to get recombinant Bacmid
  • PCR analysis
  • Isolate recombinant bacmid DNA
  • Transfect recombinant Bacmid DNA into insect cells to obtain baculovirus, and detect expression level by SDS-PAGE
  • Repeat the infection if necessary
Cusabio Features
Suspension transfection

Unique suspension transfection method greatly increases the protein expression level, and effectively shortens the experimental cycle




STEP 03 Scale up expression and purification
대규모 발현 및 정제
Process
  • Infect insect cells with appropriate baculovirus
  • The target protein is purified by affinity chromatography, ion exchange, hydrophobic and molecular sieves
Cusabio Features
Expression optimization

After optimization, the large amount of protein can be obtained by infecting host cells with low-passage virus




STEP 04 Additional Services
특수 가공 Optional
Process
  • ChargedTag removal by restriction digestion
  • Free Filter-sterilization
  • Free Endotoxin removal
  • Free Lyophilization ※ Note : Lyophilization and Filter-sterilization can not be met simultaneously
Cusabio Features
Flexible additional services

Customers can flexibly choose from a variety of additional services to their specific needs, e.g. Endotoxin removal, Filter-sterilization, Tag removal, Lyophilization, etc. Some are complimentary, and some require additional charge




STEP 05 Quality Control
품질 검증
Process
  • Testing of purity, concentration, etc.
  • QC report is provided
Cusabio Features
Detailed COA report

Detailed product data sheet and COA are provided for each project