Sanger sequencing

Regular Sequencing

  • Normal Sequencing
  • Difficult Sequencing
  • Additional Services

Special Sequencing

  • Microbial Identification
  • Full Sequencing
  • Mitochondrial DNA sequencing
  • Bisulfite sequencing
  • Fragment Analysis
  • Cell line authentication
  • NGS Integration service
  • Designed PCR Sequencing (DPS)

Universal Primer list

Vector-Binding Primer

Troubleshooting

Sanger sequencing

Cosmogenetech provides fast and accurate DNA sequencing services using a high-throughput DNA Analyzer.

Workflow

01

Sample Collection

  • Seoul, parts of Gyeonggi, and Daejeon: Direct pickup
  • Other regions: Courier or postal delivery
02

Sample Verification

  • Samples are not stored long-term. Please request sample return if needed.
03

Sample QC

  • Template DNA concentration and purity checked by agarose gel electrophoresis
04

Sequencing Reaction, Purification & Running

  • BigDye® Terminator v3.1 Cycle sequencing kit
  • Applied Biosystems 3730xl DNA Analizer
  • High-purity purification using magnetic bead method
05

Result Analysis & Delivery

  • Results delivered within 24 hours via website, E-mail, and SMS
  • (Data retention period: 6 months)

Key Features

  • Results delivered within 24 hours of sample submission, with SMS notification
  • Wide variety of universal primers provided free of charge
  • Fast results through integration with our sequencing primer synthesis service
  • Long and accurate reads using a 50 cm long capillary
  • Various additional services available in conjunction with our products and services

Service Pricing

Normal sequencing
₩7,500
Difficult sequencing
₩15,000
Full sequencing
₩60,000 / kb
BigDye pre-reacted running
₩4,000
Plasmid miniprep
₩4,500
PCR purification
₩4,500
Gel extraction
₩4,500
Custom PCR
₩3,000
  • For bulk sample pricing, please contact your sales representative.